Reagents from InVitroCare®

Reagents for ICSI (Intracytoplasmic Sperm Injection)

PVP

Ready-to-use solution of Polyvinylpyrrolidone dissolved in InVitroCare® Sperm Wash Medium and sterile filtered.

Used to slow sperm movement as well as coat the inner bore of ICSI micropipettes allowing for the smooth transfer of a spermatozoon into the ooplasm.

1ml	(2210)
1ml	(2212)
1ml	(2211)

Hyaluronidase

Ready-to-use solution consisting of 100Eu/ml or Hyaluronidase dissolved into InVitroCare® Sperm Wash Medium and sterile filtered.

Used for the removal of coronal cells adhering to the zona pellucida during ICSI procedures.

Reagents for Assisted Hatching

Acidic Tyrodes Solution

Ready-to-use solution of a pH range of 2.2 - 2.4
Used for local dissolving of the zona pellucida when used with assisted hatching micropipettes.

Mineral Oil from InVitroCare®

Pharmaceutical Grade Mineral Oil

Density:      0.83-0.86 g/ml
Viscosity:   <30 cP @ 30°C
Endotoxin:  <0.25 EU/mL

InVitroCare® Mineral Oil is pre-washed, no further preparation is needed.  Simply place opened bottle of InVitroCare® Mineral Oil in a 5-6% CO₂ incubator for 24 hours prior to use.

• Manufactured using pharmaceutical grade mineral oil
• Double pre-washed, ready to use
• Sterilized by filtration and aseptic filing
• Mouse Embryo tested (>70% blastocysts after 96 hours)
• Manufactured to meet Ph Eur, BP and USP standards
• Use offers:
• Better pH stability
• Better temperature stability
• Stable osmolarity
• Protection against contamination